3000x650_Pre-designed_panel_project_v3

Industry-standard, pre-designed panels for targeted genotyping by sequencing

LGC Biosearch Technologies' industry standard, pre-designed panels utilise well-characterised, industry-standard content to provide fast access to the targeted GBS technology - Amp-Seq One - at a lower cost. Panels can be easily and affordably modified over time, allowing for changes in marker density as well as the addition or removal of markers. This enables flexible and cost-effective customisation of the ideal panel for your breeding programme.

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Key benefits of Amp-Seq One panels

  • Unique simplicity with a 1-stage workflow
  • Same-day sequencing with a 120-minute protocol
  • High performance, featuring industry leading SNP call rates and even coverage
  • Compatible with a variety of sample types and DNA preparation methods
  • Reduced environmental impact with components stable at ambient temperatures

The first pre-designed panels available for ordering are shown below.

Amp-Seq One industry standard bovine ICAR+ 0.6K panel
Bovine
Versions
SNPline and Nexar
384 and 3840 reactions
Markers
565 markers
587 amplicons
Reads per sample
120,000 reads
(200X coverage)
Sequencing
Single end 100 bp
Compatible with longer/paired

Validated Breeds/Varieties

Marchigian, Romagnola, Holstein, Jersey, Brown Swiss, Piemontese, Podolica, Limousine, Chianina, Charolaise, Zebu-Nellore

Marker Details

  • Contains 340 ISAG markers
  • Contains 199 SNPs from ISAG panel (parentage)
  • Contains four additional traits
  • A2/A2
  • Coat color (Red/Black/Wild)
  • Pompes E7
  • Horned/Polled (Celtic and Friesian)
Marker List Data Sheet Order Panel
Amp-Seq One industry standard Maize 1.3K panel
Maize
Versions
SNPline and Nexar
384 and 3840 reactions
Markers
1291 markers
1152 amplicons
Reads per sample
250,000 reads
(200X coverage)
Sequencing
Single end 100 bp
Compatible with longer/paired

Validated Breeds/Varieties

B73

Marker Details

  • Contains 1251 markers from Axiom_Maize600
  • Contains 558 markers from Illumina MaizeSNP50 BeadChip
Marker List Please Enquire
Amp-Seq One industry standard Maize 5.9K panel
Maize
Versions
SNPline and Nexar
384 and 3840 reactions
Markers
5890 markers
5257 amplicons
Reads per sample
1,100,000 reads
(200X coverage)
Sequencing
Single end 100 bp
Compatible with longer/paired

Validated Breeds/Varieties

B73

Marker Details

  • Contains 5574 markers from Axiom_Maize600
  • Contains 2422 markers from Illumina MaizeSNP50 BeadChip
Marker List Please Enquire
Amp-Seq One industry standard bovine ISAG 0.2K panel
Bovine
Versions
SNPline and Nexar
384 and 3840 reactions
Markers
199 markers
199 amplicons
Reads per sample
40,000 reads
(200X coverage)
Sequencing
Single end 150 bp
Compatible with longer/paired

Validated Breeds/Varieties

Marchigiana, Romagnola, Holstein, Jersey, Brown Swiss, Piemontese, Podolica, Limousine, Chianina, Charolaise, Zebu-Nellore

Marker Details

  • Contains 199 SNPs from ISAG panel (parentage)
Marker List Please Enquire
* co-developed with Illumina Inc.

Panel name

Species

Versions

Breeds/varieties validated

Marker number

Amplicon number 

 Marker details

Number of reads per sample

Sequencing chemistry

More information

 Order by product no.

Amp-Seq One industry standard bovine ICAR+ 0.6K panel*

Bovine

SNPline and Nexar.
384 and 3840 reactions.

Marchigian, Romagnola, Holstein, Jersey, Brown Swiss, Piemontese, Podolica, Limousine, Chianina, Charolaise, Zebu-Nellore

565

587

 - Contains 340 ISAG markers

- Contains 199 SNPs from ISAG panel (parentage)
- Contains four additional traits
- A2/A2
- Coat color (Red/Black/Wild)
- Pompes E7
- Horned/Polled (Celtic and Friesian)

120,000 reads per sample
(based on 200X amplicon coverage rounded up)

Single end 100 bp. Longer read legths or paired end sequence are fully compatible

- Marker list
- Data sheet

GEN-9910-384-5566/
GEN-9910-3840-5566/

Specifically developed for Nexar™ instrument
GEN-9910-384N-5566
GEN-9910-3840N-5566

Amp-Seq One industry standard Maize 1.3K panel

Maize

SNPline and Nexar.
384 and 3840 reactions.

B73

1291

1152

- Contains 1251 markers from Axiom_Maize600 
- Contains 558 markers from Illumina MaizeSNP50 BeadChip

250,000 reads per sample
( based on 200X amplicon coverage rounded up)

Single end 100 bp. Longer read legths or paired end sequence are fully compatible

 - Marker list

GEN-9910-384-1291/
GEN-9910-3840-1291/
GEN-9910-384N-1291/
GEN-9910-3840N-1291
Please enquire below

Amp-Seq One industry standard Maize 5.9K panel

Maize

SNPline and Nexar.
384 and 3840 reactions.

B73

5890

5257

- Contains 5574 markers from Axiom_Maize600 
- Contains 2422 markers from Illumina MaizeSNP50 BeadChi

1,100,000 reads per sample
(based on 200X amplicon coverage rounded up)

Single end 100 bp. Longer read legths or paired end sequence are fully compatible

 - Marker list

GEN-9910-384-5890/
GEN-9910-3840-5890/
GEN-9910-384N-5890/
GEN-9910-3840N-5890
Please enquire below

Amp-Seq One industry standard bovine ISAG 0.2K panel

Bovine

SNPline and Nexar.
384 and 3840 reactions.

Marchigiana, Romagnola, Holstein, Jersey, Brown Swiss, Piemontese, Podolica, Limousine, Chianina, Charolaise,
Zebu-Nellore

199

199

- Contains 199 SNPs from ISAG panel (parentage)

40,000 reads per sample
(based on 200X amplicon coverage rounded up)

Single end 150 bp. Longer read legths or paired end sequence are fully compatible

 - Marker list

GEN-9910-384-5199/
GEN-9910-3840-5199/
GEN-9910-384N-5199/
GEN-9910-3840N-5199
Please enquire below
 
* co-developed with Illumina Inc.

Additional resources

Amp-Seq One panel data sheet

Amp-Seq One bovine ICAR+ panel data sheet

Download
Amp-Seq One

Amp-Seq One information

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Flex-Seq

Flex-Seq (service)

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