Industry-standard, pre-designed panels for targeted genotyping by sequencing

LGC Biosearch Technologies' industry standard, pre-designed panels utilise well-characterised, industry-standard content to provide fast access to the targeted GBS technology - Amp-Seq One - at a lower cost. Panels can be easily and affordably modified over time, allowing for changes in marker density as well as the addition or removal of markers. This enables flexible and cost-effective customisation of the ideal panel for your breeding programme.

Key benefits of Amp-Seq One panels

Unique simplicity with a 1-stage workflow
Same-day sequencing with a 120-minute protocol
High performance, featuring industry leading SNP call rates and even coverage
Compatible with a variety of sample types and DNA preparation methods
Reduced environmental impact with components stable at ambient temperatures

The first pre-designed panels available for ordering are shown below.

Panel name	Species	Versions	Breeds/varieties validated	Marker number	Amplicon number	Marker details	Number of reads per sample	Sequencing chemistry	More information	Order by product no.
Amp-Seq One industry standard bovine ICAR+ 0.6K panel*	Bovine	SNPline and Nexar. 384 and 3840 reactions.	Marchigian, Romagnola, Holstein, Jersey, Brown Swiss, Piemontese, Podolica, Limousine, Chianina, Charolaise, Zebu-Nellore	565	587	- Contains 340 ISAG markers - Contains 199 SNPs from ISAG panel (parentage) - Contains four additional traits - A2/A2 - Coat color (Red/Black/Wild) - Pompes E7 - Horned/Polled (Celtic and Friesian)	120,000 reads per sample (based on 200X amplicon coverage rounded up)	Single end 100 bp. Longer read legths or paired end sequence are fully compatible	- Marker list - Data sheet	GEN-9910-384-5566/ GEN-9910-3840-5566/ Specifically developed for Nexar™ instrument GEN-9910-384N-5566 GEN-9910-3840N-5566
Amp-Seq One industry standard Maize 1.3K panel	Maize	SNPline and Nexar. 384 and 3840 reactions.	B73	1291	1152	- Contains 1251 markers from Axiom_Maize600 - Contains 558 markers from Illumina MaizeSNP50 BeadChip	250,000 reads per sample ( based on 200X amplicon coverage rounded up)	Single end 100 bp. Longer read legths or paired end sequence are fully compatible	- Marker list	GEN-9910-384-1291/ GEN-9910-3840-1291/ GEN-9910-384N-1291/ GEN-9910-3840N-1291 Please enquire below
Amp-Seq One industry standard Maize 5.9K panel	Maize	SNPline and Nexar. 384 and 3840 reactions.	B73	5890	5257	- Contains 5574 markers from Axiom_Maize600 - Contains 2422 markers from Illumina MaizeSNP50 BeadChi	1,100,000 reads per sample (based on 200X amplicon coverage rounded up)	Single end 100 bp. Longer read legths or paired end sequence are fully compatible	- Marker list	GEN-9910-384-5890/ GEN-9910-3840-5890/ GEN-9910-384N-5890/ GEN-9910-3840N-5890 Please enquire below
Amp-Seq One industry standard bovine ISAG 0.2K panel	Bovine	SNPline and Nexar. 384 and 3840 reactions.	Marchigiana, Romagnola, Holstein, Jersey, Brown Swiss, Piemontese, Podolica, Limousine, Chianina, Charolaise, Zebu-Nellore	199	199	- Contains 199 SNPs from ISAG panel (parentage)	40,000 reads per sample (based on 200X amplicon coverage rounded up)	Single end 150 bp. Longer read legths or paired end sequence are fully compatible	- Marker list	GEN-9910-384-5199/ GEN-9910-3840-5199/ GEN-9910-384N-5199/ GEN-9910-3840N-5199 Please enquire below

* co-developed with Illumina Inc.

Additional resources

Industry-standard, pre-designed panels for targeted genotyping by sequencing

Additional resources

Amp-Seq One bovine ICAR+ panel data sheet

Amp-Seq One information

Flex-Seq (service)