RT-qPCR is a well-established method with an equally well-established history of optimisation challenges. If your lab runs RT-qPCR assays, you have likely spent time troubleshooting amplifications that produce unexpected curves, irreproducible data or a later than expected CT value. When it comes to SARS-CoV-2 detection assays, the pressure for consistent, reproducible results is high - as is the volume of samples. Follow our expert tips to maximise data quality in a high-throughput, high pressure diagnostic lab.