7 tips to maximise RT-qPCR success for SARS-CoV-2 detection

Expert guidance to ensure your assay is consistent, accurate and scalable


From workspace workflow to data analysis, see where you have opportunities to optimise your RT-qPCR detection setup.

RT-qPCR is a well-established method with an equally well-established history of optimisation challenges. If your lab runs RT-qPCR assays, you have likely spent time troubleshooting amplifications that produce unexpected curves, irreproducible data or a later than expected CT value. When it comes to SARS-CoV-2 detection assays, the pressure for consistent, reproducible results is high - as is the volume of samples. Follow our expert tips to maximise data quality in a high-throughput, high pressure diagnostic lab.