Accelerate your journey
With agricultural genotyping products and services
With agricultural genotyping products and services
Monday, 16 January | 12:50 PM - 3:00 PM | Town & Country B
Join Zander Myburg from the University of Pretoria Forest and Agricultural Biotechnology Institute (FABI), Dr Shaun Clare, Research Geneticist for the USDA-ARS National Germplasm Laboratory and Dr Johnathon LaMantia Manager of Tree R&D for Terviva talking about the genomic technologies that enable innovation and creativity to address modern agricultural hurdles. Scientists looking into the future to create crop breeding solutions for agriculture impacted by a changing climate, water scarcity, less arable land to meet production needs, supply chain disruptions, production instabilities and a limited labour force, all of which affect farmer's ability to meet the need for an increased food supply to feed a growing population.
Hear about:
Add to Calendar
Professor in Genetics,
University of Pretoria
Zander Myburg is a Professor in Genetics and Director of the Forest Molecular Genetics (FMG) Programme in the Forestry and Agricultural Biotechnology Institute (FABI) at the University of Pretoria (UP). His research team has pioneered the use of population genomics and systems genetics approaches to unravel the genetic control of growth and wood formation in Eucalyptus trees. He was the lead investigator of the US Department of Energy (DOE) Eucalyptus Genome Project which generated the reference sequence for the genus (Myburg et al. 2014, Nature) and a new, ongoing DOE-funded project for large-scale sequencing of over 3700 eucalypt genomes (2022-2026).
Manager, Tree R&D,
Terviva
Manager, Tree R&D, Terviva
Jonathan La Mantia received a BS in environmental plant biology from the University of Rhode Island and a PhD in ecological and molecular plant physiology from the Pennsylvania State University. Jonathan is the manager of germplasm analytics at Terviva and is developing genomics tools to build a molecular breeding program for pongamia. Jonathan has extensive research experience developing genomic resources and elucidating the genetic mechanisms that help regulate yield, seed composition and pest resistance in soybeans, trees and grasses. He is recognized as the scientist who discovered the dwarfing mechanism in annual bluegrass. More recently, he identified new regions in the soybean genome that regulate seed protein and oil, soybean aphid resistant and seed coat hardness.
In addition to the workshop, attendees can interact with experts during our poster presentations featuring minor groove binder (MGB) probes and an assay platform used for high-throughput genotyping for complex plant species. Our second poster focuses on a new targeted genotyping by sequencing technology (tGBS) for high-throughput agricultural parentage, marker-assisted selection, and genomic selection programs.
Poster #PE0056
A rapid and simple solution with a
streamlined workflow for
high-throughput multiplex genotyping
16 January @ 10:00 AM - 11:30 AM
High throughput, multiplex genotyping solutions are in high demand for agricultural parentage, marker-assisted selection, and genomic selection programs. Targeted sequencing technologies can enable simultaneous genotyping of hundreds to thousands of loci per sample, with a throughput of thousands of samples per sequencing run. Here we describe an extremely simple, robust targeted genotyping technology. The unique library construction chemistry and rigorous panel design processes combine to provide for consistent genotype calling and high evenness of target coverage, ensuring economy of sequencing costs. We have tested this new technology with a variety of panel sizes on different crops and animal species with simple to complex polyploid genomes, demonstrating its power and flexibility.
Poster #PE0074
A combination of MGB probes and BHQ
Probe Master Mix provides an assay
platform compatible with high-throughput automation for quick and accurate
genotyping of complex plant species
using crude DNA extracts
16 January @ 3:00 PM - 4:00 PM
The addition of a minor groove binding (MGB) moiety is well-established as a chemistry for the design of short oligonucleotide probes, due to its inherent ability to stabilise the target-probe duplex, increasing Tm, which in turn increases target hybridisation specificity. As a result, MGB probes are chosen for genotyping and qPCR applications in both the AgBio and MDx fields. Here, LGC Biosearch Technologies present our proprietary MGB probes for testing a variety of plant species (genotyping of wheat and maize and qPCR for soy GMO trait identification), illustrating our complete workflow processes from nucleic acid extraction using HotShot prior to genotyping determination on our high-throughput automation platforms. We demonstrate excellent performance of our MGB probes with crude DNA, with clear genotyping clusters easily assigned using our BHQ Probe Master Mix and three of our automation platforms, SNPline™, Nexar™ and IntelliQube™. The use of our MGB probes is clearly described in a range of efficient, cost-competitive high-throughput solutions for AgBio genotyping, using a PCR master mix which has been highly optimised for both genotyping and qPCR applications.