Multiplex qPCR is a popular area of interest for many assay developers. Thanks to distinct reporter dyes, multiple reactions can be combined into a single well, with each target detected independently. This means a single reaction can yield additional data, while reducing the amount of sample required and reagent costs.

However, multiplex assays are inherently more complicated than singleplex, making it crucial to carefully identify the optimal conditions. There is a risk of interactions between multiple primers and probes, or that some reagents may be exhausted quicker than others, leading to unbalanced results.

This ebook is packed with tips covering:

• Design considerations unique to multiplex assays
• Ensuring all singleplex assays are performing reliably
• Common challenges and solutions for optimising in multiplex
• Validation and quality control
  
  

Get the ebook and start enhancing your multiplex assays