Typically, RNA templates that have complex secondary structure require a completely separate high temperature, pre-denaturation step and cold snap step before RT-qPCR. The reverse transcriptase in RapiDxFire Lyo-Flex enables sensitive detection without a separate RNA denaturation step.
Unlike other RTs, the RT in RapiDxFire Lyo-Flex works at temperatures above 70 °C and remains stable at 85 °C for several minutes.
The graphs show exceptional detection of rotavirus type A. The dsRNA rotavirus was added directly to the reaction with RapiDxFire Lyo-Flex (top) and with Competitor B (bottom). Each was amplified per the vendor’s standard protocol.
Leverage RapiDxFire Lyo-Flex as you develop point of care pathogen detection tests. This master mix boasts a 5x concentration, which allows for smaller lyobeads with more room for excipients.
We worked with a lyophilisation CRO to demonstrate lyo-compatibility. The CRO prepared lyobeads with RapiDxFire Lyo-Flex and excipients. Then they performed a reaction to detect multiple targets first using the liquid master mix (A) and then using reconstituted lyobeads (B). RapiDxFire Lyo-Flex demonstrated accurate, sensitive detection pre- and post-lyophillisation.