Ready to order RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix?

Existing online customers of Lucigen
New online customers of LGC Biosearch Technologies
50% off your first 1 mL

RapiDxFire Lyo-Flex is ready for your workflow

with a unique set of features selected with input from top MDx manufacturers

 

150x150_icon_difficult_templates_purple-1

The unique heat-activated thermostable reverse transcriptase can withstand cDNA synthesis at high temperatures, enabling detection of previously elusive viruses. The master mix can detect RNA and DNA templates, making it ideal for viruses as well as bacteria. 

150x150_icon_lyo_compatible_purple

Lyophilise this master mix and bring PCR-based pathogen detection out of the lab. 

150x150_icon_mulitplex_purple

With an abundance of key PCR reaction components and a wide dynamic range, this master mix supports detection of multiple pathogens in a single reaction.

150x150_icon_room-temp_purple

The master mix remains stable in a broad range of temperatures and conditions, giving you more flexibility in shipping, storage and automation set up.

150x150_icon_5x_concentration_purple

5x concentration creates opportunity for higher test sensitivity while reducing waste.

 

RapiDxFire Lyo-Flex is a general purpose reagent manufactured in an ISO 13485-certified facility.

 

Bring RT-qPCR out of the lab

With our lyo-compatible, single tube, 1-step master mix

Figure 4 - Option 3 - pre post lyo

Leverage RapiDxFire Lyo-Flex as you develop point of care pathogen detection tests. This master mix boasts a 5x concentration, which allows for smaller lyobeads with more room for excipients. 

We worked with a lyophilisation CRO to demonstrate lyo-compatibility. The CRO prepared lyobeads with RapiDxFire Lyo-Flex and excipients. Then they performed a reaction to detect multiple targets first using the liquid master mix (A) and then using reconstituted lyobeads (B).  RapiDxFire Lyo-Flex demonstrated accurate, sensitive detection pre- and post-lyophillisation. 

Sensitive detection of difficult templates

Picture1

With our unique heat-activated thermostable reverse transcriptase

Typically, RNA templates that have complex secondary structure require a completely separate high temperature, pre-denaturation step and cold snap step before RT-qPCR. The reverse transcriptase in RapiDxFire Lyo-Flex enables sensitive detection without a separate RNA denaturation step.

Unlike other RTs, the RT in RapiDxFire Lyo-Flex works at temperatures above 70 °C and remains stable at 85 °C for several minutes. 

The graphs show exceptional detection of rotavirus type A. The dsRNA rotavirus was added directly to the reaction with RapiDxFire Lyo-Flex (top) and with Competitor B (bottom). Each was amplified per the vendor’s standard protocol.