--- title: Lyo-compatible RT-qPCR master mix description: Designed with input from top MDx assay manufacturers, RapiDxFire Lyo-Flex supports the development of diagnostic devices and assays that meet healthcare provider and consumer demand for fast, accurate results. With consistent results in a liquid state at room temp or in a lyophilised state, this master mix will help you achieve predictable performance in unpredictable conditions. --- [![Biosearch-White_Blue-O](https://info.biosearchtech.com/hubfs/Logos/Biosearch-White_Blue-O.png) ](https://www.biosearchtech.com/) [Login](https://info.biosearchtech.com/) [ Register](https://info.biosearchtech.com/) [Quick Order](https://info.biosearchtech.com/) ## The future requires flexibility Find your MDx assay's future flex with the new RapiDxFire Lyo-Flex RT-qPCR master mix [Product details](https://info.biosearchtech.com/lyo-flex-rt-qpcr-master-mix#product) # RapiDxFire Lyo-Flex is ready for your workflow ### with a unique set of features selected with input from top **MDx manufacturers**   | ![150x150_icon_difficult_templates_purple-1](https://info.biosearchtech.com/hs-fs/hubfs/150x150_icon_difficult_templates_purple-1.png?width=75&name=150x150_icon_difficult_templates_purple-1.png) | The **unique heat-activated thermostable reverse transcriptase** can withstand cDNA synthesis at high temperatures, enabling detection of previously elusive viruses. The master mix can **detect RNA and DNA templates**, making it ideal for viruses as well as bacteria.  | | --- | --- | | ![150x150_icon_lyo_compatible_purple](https://info.biosearchtech.com/hs-fs/hubfs/150x150_icon_lyo_compatible_purple.png?width=75&name=150x150_icon_lyo_compatible_purple.png) | **Lyophilise** this master mix and bring PCR-based pathogen detection out of the lab.  | | ![150x150_icon_mulitplex_purple](https://info.biosearchtech.com/hs-fs/hubfs/150x150_icon_mulitplex_purple.png?width=75&name=150x150_icon_mulitplex_purple.png) | With an abundance of key PCR reaction components and a wide dynamic range, this master mix supports **detection of multiple pathogens in a single reaction**. | | ![150x150_icon_room-temp_purple](https://info.biosearchtech.com/hs-fs/hubfs/150x150_icon_room-temp_purple.png?width=75&name=150x150_icon_room-temp_purple.png) | The master mix remains **stable in a broad range of temperatures** and conditions, giving you more flexibility in shipping, storage and automation set up. | | ![150x150_icon_5x_concentration_purple](https://info.biosearchtech.com/hs-fs/hubfs/150x150_icon_5x_concentration_purple.png?width=75&name=150x150_icon_5x_concentration_purple.png) | **5x concentration** creates opportunity for higher test sensitivity while reducing waste. | *RapiDxFire Lyo-Flex is a general purpose reagent manufactured in an ISO 13485-certified facility.*   ### [![Order now](https://no-cache.hubspot.com/cta/default/54061/f8f673eb-83fa-4f74-92c9-ff196722809a.png)](https://cta-redirect.hubspot.com/cta/redirect/54061/f8f673eb-83fa-4f74-92c9-ff196722809a) # Sensitive detection of difficult templates # ![Picture1](https://info.biosearchtech.com/hs-fs/hubfs/Picture1.png?width=355&name=Picture1.png) ### With our unique heat-activated thermostable reverse transcriptase Typically, RNA templates that have complex secondary structure require a completely separate high temperature, pre-denaturation step and cold snap step before RT-qPCR. The reverse transcriptase in RapiDxFire Lyo-Flex enables sensitive detection without a separate RNA denaturation step. Unlike other RTs, the RT in RapiDxFire Lyo-Flex works at temperatures above 70 °C and remains stable at 85 °C for several minutes.  The graphs show exceptional detection of rotavirus type A. The dsRNA rotavirus was added directly to the reaction with RapiDxFire Lyo-Flex (top) and with Competitor B (bottom). Each was amplified per the vendor’s standard protocol. [ Order now ](https://shop.biosearchtech.com/pcr-instruments%2c-reagents-and-consumables/pcr-and-qpcr-reagents/pcr-and-qpcr-master-mixes/rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix/p/PCRAMP-021) # Bring RT-qPCR out of the lab ### With our lyo-compatible, single tube, 1-step master mix ### ![Figure 4 - Option 3 - pre post lyo](https://info.biosearchtech.com/hs-fs/hubfs/Figure%204%20-%20Option%203%20-%20pre%20post%20lyo.png?width=500&name=Figure%204%20-%20Option%203%20-%20pre%20post%20lyo.png) Leverage RapiDxFire Lyo-Flex as you develop point of care pathogen detection tests. This master mix boasts a 5x concentration, which allows for smaller lyobeads with more room for excipients.  We worked with a lyophilisation CRO to demonstrate lyo-compatibility. The CRO prepared lyobeads with RapiDxFire Lyo-Flex and excipients. Then they performed a reaction to detect multiple targets first using the liquid master mix (A) and then using reconstituted lyobeads (B).  RapiDxFire Lyo-Flex demonstrated accurate, sensitive detection pre- and post-lyophillisation.  [ Order now ](https://shop.biosearchtech.com/pcr-instruments%2c-reagents-and-consumables/pcr-and-qpcr-reagents/pcr-and-qpcr-master-mixes/rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix/p/PCRAMP-021) --- Connect with us [Contact us](https://www.biosearchtech.com/about-us/locations) [Privacy Notice](https://www.biosearchtech.com/about-us/legal/privacy) Follow us [**](https://www.linkedin.com/company/lgc-biosearch-technologies) [** ](https://twitter.com/LGCBiosearch) [** ](https://www.facebook.com/LGCBiosearch) [** ](https://www.youtube.com/lgcgroupvideo) [![Biosearch Technologies logo grey](https://info.biosearchtech.com/hs-fs/hubfs/Biosearch%20Technologies%20logo%20grey.png?width=180&height=51&name=Biosearch%20Technologies%20logo%20grey.png "Biosearch Technologies logo grey")](https://www.biosearchtech.com) ![FINAL LGC NEW LOGO - cmyk_White (1)](https://info.biosearchtech.com/hs-fs/hubfs/FINAL%20LGC%20NEW%20LOGO%20-%20cmyk_White%20(1).png?width=48&height=48&name=FINAL%20LGC%20NEW%20LOGO%20-%20cmyk_White%20(1).png "FINAL LGC NEW LOGO - cmyk_White (1)") Copyright © 2023 LGC Biosearch Technologies