How to pick the right probe for your qPCR assay

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Today, qPCR is the most popular method for quantifying nucleic acids. The technique is easy to perform and offers excellent detection sensitivity, but how do you pick the right probe for your assay?

While most probes will deliver some degree of result for most applications, selecting the best probe for a given application is instrumental to assay optimisation.

This technical guide includes details on how to:

  • How to select the right probe based on sample characteristics.
  • Tips and tricks for matching probe design with downstream assays.
  • How to troubleshoot probe-based qPCRs.

Also included is a summary of our industry-leading qPCR probe portfolio, each with their own qualities and advantages to guide your selection process.

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