Get the competitive edge

Launching a qPCR assay requires deep expertise, access to tools supporting different probe chemistries and a lot of time to go through huge volumes of published qPCR data to select the best experimental framework.

If you're after the highest possible quality qPCR assay, but lack time and access to certain tools or have a challenging target, then you will benefit from this guide. 

This e-book includes advice on:

• Performing in silico validation to assess and optimise primer and probe design and selection
• Sample extraction, processing and implementing controls for your assay
• Selecting an optimal oligo concentration, cycling conditions and MgCI₂ concentration
• Calculating and evaluating key performance parameters, generating a standard curve and assessing efficiency
• Identifying sources of potential variation, implementing normalisation steps and selecting a quantification method
• Analysing, gathering and reporting experimental data
• And more...

Get your free copy today by completing this form.