Accelerate your breeding programme

Gain a competitive edge in your breeding program, and get actionable data to make decisions faster. KASP is an easy-to-use, affordable and scalable genotyping method yielding highly reproducible results, even on complex or highly repetitive genomes.

KASP is trusted by world-class breeding programs:

  • Critical component of breeding programs around the globe for over 15 years.
  • Easy-to-implement technology is used on every content, on hundreds of species.
  • Cited in over 2,500 publications, and growing daily.

Want to try KASP in your lab?

Order your free KASP trial kit here

Trusted technology, trusted partner

LGC Biosearch Technologies is the only provider of the original patented KASP technology, and your sole partner offering complete workflow solutions built specifically for agrigenomics – including automated sample extraction, large-scale liquid dispensing and PCR instrumentation, software, reagents, complete genotyping services, best-in-class technical support and ISO9001-certified manufacturing.

Why use KASP?

See the advantages KASP brings to your breeding programme:

Superb accuracy and performance

  • Accuracy >99.8%*
  • Industry leading SNP & InDel assay conversion rate (>90%)
  • Now without Triton X-100 in compliance with the REACH Regulation

(* based on independent assessment)

Easy to use

  • Requires only three simple components: KASP-TF Master Mix, KASP Assay Mix designed for your specific target, and sample DNA.
  • Flexible primer design combined with Biosearch’s 15+ years of expertise means successful assay design for your SNP, Indel or Mega Indel.
  • Supports low-, medium- and high-throughput studies and individual repeat assays
  • Signal can be read on most FRET-capable plate readers and qPCR instruments.

Affordable

  • Eliminates the need for expensive labelled assay-specific primers or probes
  • Compatible with crude DNA extraction methods
  • Low reaction volumes – down to 1 uL - keep reagent costs to a minimum
  • Highly accurate, robust assay means less time spent on repeats
  • Cost benefits enable you to perform more assays overall, improving the quality of your data.

Ready to see for yourself?

Order your KASP trial kit here
KASP_genotyping_reaction

How does KASP work?

Enables bi-allelic scoring of SNPs and Indels.

KASP genotyping technology is based on competitive allele-specific PCR and enables bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci.

KASP is easy to use – simply add your SNP-specific KASP Assay Mix and the universal KASP-TF Master Mix to your DNA samples, run a thermal cycling program, and measure the end-point fluorescence.

How_KASP_works_graphic_only
The KASP Assay mix contains three assay-specific non-labelled oligos: two allele-specific forward primers and one common reverse primer.

The allele-specific primers each harbour a unique tail sequence that corresponds with a universal FRET (fluorescence resonant energy transfer) cassette; one labelled with FAM™ dye and the other with HEX™ dye. The KASP-TF Master Mix contains the universal FRET cassettes, ROX™ passive reference dye, taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution.

 

KASP is the affordable solution

A key feature of our PCR-based KASP technology is the use of a universal FRET cassette reporter system that eliminates the need for costly dual-labelled probes. Our allele-specific forward primers each have a proprietary tail sequence that corresponds with one of two FRET cassettes: one label with FAM dye and the other with HEX dye. This technology gives KASP a clear cost advantage, and when combined with miniaturised reaction volumes (10 µL in 96-well PCR plates, 5 µL in 384-well PCR plates; 1 µL in 1536-well PCR plates), delivers an exceptionally accurate, flexible, and cost-effective genotyping solution. Bi-allelic discrimination is achieved through the competitive binding of the two allele-specific forward primers.

Biosearch Technologies is your ideal partner for KASP high-throughput genotyping

  • Work with our experts. Biosearch Technologies brings over 15 years of expertise in high throughput genotyping with gold-standard KASP genotyping technology.
  • Trust our quality. Each batch of KASP-TF Master Mix is made in our ISO9001-compliant manufacturing facility and functionally tested in our high throughput genotyping facility.
  • Let us support you. Global teams of sales, customer service and technical support specialists provide regional support for your genotyping program.
  • Complete workflow solutions include high-throughput sample extraction and genotyping instrumentation built specifically for agrigenomics workflows, along with software and support packages, and fully validated protocols.
  • Reliable supply. Our stable supply chain, including disaster recovery processes, allows consistent large-scale order delivery.
  • Complete genotyping services that can complement your in-house genotyping and handle your seasonal overflow.

Ordering

Ready to order? KASP-TF Master Mix can be ordered independently, or in conjunction with your KASP Assay Mix. Order your KASP genotyping components here:

Or, visit our Complete Genotyping Services page to learn how we can partner with you.

  • All-inclusive services

Resources

Copyright © 2000-2020 LGC Biosearch Technologies